mouse cell lines human breast cancer cell lines  (ATCC)

Journal: bioRxiv

Article Title: Engineered probiotics for local tumor delivery of checkpoint blockade nanobodies

doi: 10.1101/562785

Figure Lengend Snippet: (a) BALB/c mice were implanted subcutaneously with 5×10 6 CT26 cells on both hind flanks and injected with strains genomically expressing a luminescence reporter for more feasible tracking of bacterial population dynamics over time. (b) Mice were dosed with 5×10 6 bacteria of either the non-lysing EcN-lux or SLC-int:PD-L1nb strain and bacterial populations were tracked using IVIS. (c) Mean relative tumor trajectory of mice receiving intratumoral injections of 5×10 6 bacteria of either SLC-int:PD-L1nb or SLC-p15A:PD-L1nb. Arrows indicate days of intratumoral injection (N=6 tumors per group, ** P=0.0065, two-way ANOVA with Bonferroni post-test, error bars represent S.E.M) (d) The absolute slope between day 0 and 6 and between day 7 and 14 (* P = 0.0175, segmented linear regression analysis). (e) Mean relative tumor trajectory of mice receiving intratumoral injections of 5×10 6 bacteria of EcN-lux or SLC-int:PD-L1nb or intraperiotoneal injection of 200ug/mouse of 10F.9G2 mAb. All treatments were administered on day 0 and 4 (N=6-8 tumors per group, ** P=0.0015. two-way ANOVA with Bonferroni post-test, error bars represent S.E.M). (f) Dirty necrosis scores of histology tissue samples from tumors treated with PBS, EcN-lux, SLC-int:PD-L1nb, and anti-PD-L1 mAb (N=6-8 scores per group of biological replicates, ***P=0.007 Mann Whitney Test ordinal non parametric between SLC-int:PD-L1nb and anti-PD-L1 mAb, error bars represent S.E.M).

Article Snippet: Animal experiments were performed on 6-8 week old female BALB/C mice from Taconic with bilateral subcutaneous hind flank tumors from an implanted mouse colorectal cancer cell line CT26, mouse lymphoma cell line A20, or a mouse breast cancer cell line, 4T1.

Techniques: Injection, Expressing, MANN-WHITNEY

Journal: bioRxiv

Article Title: Engineered probiotics for local tumor delivery of checkpoint blockade nanobodies

doi: 10.1101/562785

Figure Lengend Snippet: BALB/c mice were implanted subcutaneously with 5×10 6 4T1 cells on both hind flanks. When tumors reached ~200mm 3 , mice received intratumoral injections of 5×10 6 bacteria every 3-4 days of either EcN-lux, SLC-PD-L1nb, or 200ug/mouse of 10F.9G2 mAb (a) Survival of mice following 5×10 6 bacteria dosed on days 0, 5, and 8 days post treatment, and systemically delivery mAb dosed on days 0, 5, 8 and 12. Severe toxicities in weight and body conditions of antibody treated mice were observed, leading to death or required euthanasia of all mice within two weeks. (b) Mean relative body weight. (c) Distribution of tumor sizes. N=6-8 per group, *P = 0.0199, two-way ANOVA with Bonferroni post-test, error bars represent S.E.M.

Article Snippet: Animal experiments were performed on 6-8 week old female BALB/C mice from Taconic with bilateral subcutaneous hind flank tumors from an implanted mouse colorectal cancer cell line CT26, mouse lymphoma cell line A20, or a mouse breast cancer cell line, 4T1.

Techniques:

Journal: bioRxiv

Article Title: Engineered probiotics for local tumor delivery of checkpoint blockade nanobodies

doi: 10.1101/562785

Figure Lengend Snippet: (a) Plasmid map for the constitutive expression of the CTLA-4 Nb on a high copy plasmid. (b) BALB/c mice were implanted subcutaneously with 5×10 6 CT26 cells on both hind flanks. When tumors reached ~200mm 3 , mice received intratumoral injections every 3-4 days of 5×10 6 bacteria of either EcN-lux, SLC-int:CTLA-4nb or 100ug/mouse intraperitoneal injections of a 9D9 mAb. Graph shows mean relative tumor trajectories. (c) Mean relative body weight. N=6-7 per group, *P=0.0407, two-way ANOVA with Bonferroni post-test, error bars represent S.E.M. (d) Cytokine levels of tumor lysates measured by Luminex Multiplex Assay. N=3 biological replicates per group, *P=0.0224, one-way ANOVA with Bonferroni post-test, error bars represent S.E.M.

Article Snippet: Animal experiments were performed on 6-8 week old female BALB/C mice from Taconic with bilateral subcutaneous hind flank tumors from an implanted mouse colorectal cancer cell line CT26, mouse lymphoma cell line A20, or a mouse breast cancer cell line, 4T1.

Techniques: Plasmid Preparation, Expressing, Luminex, Multiplex Assay